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KMID : 1134120060090010014
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Journal of Breast Cancer
2006 Volume.9 No. 1 p.14 ~ p.17
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Mutational Analysis of 17? -hydroxysteroid dehydrogenase type 2 gene in Breast Cancers
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Cho Min-Sun
Kim Hyun-Ah
Lee Shi-Nae
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Abstract
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Purpose: The 17?-hydroxysteroid dehydrogenases(17HSDs) play an important role in the regulation of the physiologic activities of sex steroid hormones. The predominance of 17HSD type 1 in the malignant breast tissue could increase the estrogen-dependent proliferation and stimulate the cancer progression. On the other hand, the oxidative 17HSD type 2 may protect the normal breast cells from an excessive estradiol effect. To identify the role of 17HSD type 2 in the carcinogenesis of breast cancer, we investigate the mutation of 17HSD type 2 in 35 breast cancers.
Methods: We analyzed the entire coding region of the 17 HSD type 2 gene for detection of the somatic mutations in 35 invasive ductal carcinomas of the breast by polymerase chain reaction, single strand conformation polymorphism, and DNA sequencing.
Results: We found one missense mutation in exon 6(2.86%). It revealed the CCT→CTT (Pro→Leu) transition type at codon 262 in exon 6.
Conclusion: In present study, we found only a mutation of the 17? -HSD type 2 gene in breast cancer and could not demonstrate the direct relationship between the mutation of the 17? -HSD type 2 gene and the development of breast cancer. These results suggest that the mutation of 17HSD type 2 doesn? play a major role in the development of breast cancer. (J Breast Cancer 2006;9: 14-17)
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KEYWORD
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Breast cancer, 17HSD type 2
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